Main Ingredients Research Articles

Rhein suppresses esophageal cancer development by regulating cell cycle through DNMT3B gene.

The mechanism by which DNMT3B facilitates esophageal cancer (ESCA) progression is currently unknown, despite its association with adverse prognoses in several cancer types. To investigate the potential therapeutic effects of the Chinese herbal medicine rhubarb on esophageal cancer (ESCA), we adopted an integrated bioinformatics approach. Gene Set Enrichment Analysis (GSEA) was first utilized to screen active anti-ESCA components in rhubarb. We then employed Weighted Gene Co-expression Network Analysis (WGCNA) to identify key molecular modules and targets related to the active components and ESCA pathogenesis. This system-level strategy integrating multi-omics data provides a powerful means to unravel the molecular mechanisms underlying the anticancer activities of natural products, like rhubarb. To investigate module gene functional enrichment, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted. In addition, we evaluated the predictive impact of DNMT3B expression on ESCA patients utilizing the Kaplan-Meier method. Finally, we conducted experiments on cell proliferation and the cell cycle to explore the biological roles of DNMT3B. In this study, we identified Rhein as the main active ingredient of rhubarb that exhibited significant anti-ESCA activity. Rhein markedly suppressed ESCA cell proliferation. Utilizing Weighted Gene Co-expression Network Analysis (WGCNA) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, we determined that the blue module was associated with Rhein target genes and the cell cycle. Additionally, DNMT3B was identified as a Rhein target gene. Analysis of The Cancer Genome Atlas (TCGA) database revealed that higher DNMT3B levels were associated with poor prognosis in ESCA patients. Furthermore, Rhein partially reversed the overexpression of DNMT3B to inhibit ESCA cell proliferation. In vitro studies demonstrated that Rhein and DNMT3B inhibition disrupted the S phase of the cell cycle and affected the production of cell cycle-related proteins. In this study, we found that Rhein exerts its anti-proliferative effects in ESCA cells by targeting DNMT3B and regulating the cell cycle.

Establishment of chemical fingerprint and determination of main active ingredients in Centellae Herba by HPLC combined with multivariate chemometrics analysis

AbstractIn this paper, a simple and efficient high‐performance liquid chromatography method was established to analyze the chemical composition fingerprint of Centellae Herba (CHA) and determine five triterpenoid active components in CHA, namely, asiaticoside B (AB), madecassoside, asiaticoside, madecasic acid, and asiaticoic acid. The separation of the compounds was carried out on an XD‐C18 column (250 mm × 4.6 mm, 5 µm), the wavelength of the ultraviolet detector was set to 205 nm, and the mobile phase was composed of acetonitrile −0.05% phosphoric acid, 2 mmol/L β‐cyclodextrin aqueous solution, and gradient elution at the flow rate of 1.0 mL/min. A general chromatographic fingerprint consisting of 20 characteristic peaks of 18 batches of CHA samples was established, and the samples were classified and evaluated by similarity analysis, hierarchical cluster analysis, and principal component analysis combined with multivariate econometric analysis. In quantitative analysis, all calibration curves showed good linear regression in the test range (r > 0.999), the average recovery was 96.12%–104.63%, and the relative standard deviations of repeatability and stability were less than 2.00%. The results show that the method is accurate and effective and can be used for the comprehensive quality control of CHA.

In vitro microrhizome induction in Acorus calamus L., a commercially important aromatic medicinal plant

Acorus calamus L., is a leading aromatic medicinal plant that produces pungent aromatic rhizomes that are valued worldwide as an important herbal medicine and is one of the main ingredients in several polyherbal formulations used for neurological and metabolic disorders. The present investigation aims to develop an efficient protocol for in vitro microrhizome induction in A. calamus for the large-scale production of disease-free planting material for commercial purposes. In vitro derived shoots were initiated on MS medium supplemented with different concentrations of sucrose alone (3–10 %) and different concentrations of sucrose (6 % and 7 %) with varying concentrations of BAP alone (0.5–2 mg/L) and combinations with IAA (0.5 and 1 mg/L) and NAA (0.5 and 1 mg/L) were carried out for the microrhizome induction experiments. In different concentrations of sucrose used, healthy and disease-free microshoots of A. calamus were obtained in MS media supplemented with 7 % sucrose followed by 6 % sucrose. The highest shoot length (24.79 ± 0.03 cm) and microrhizome size (4.98 ± 0.03 cm length and 399.60 ± 0.37 mg fresh weight) were obtained in MS solid medium supplemented with 1 mg/L BAP and 0.5 mg/L NAA with 7 % sucrose followed by the same hormone concentration with 6 % sucrose (shoot length-23.68 ± 0.03 cm, microrhizome length- 4.68 ± 0.03 cm and fresh weight of microrhizome-376.60 ± 0.57 mg). The developed protocol can be used for large-scale production of disease-free propagules without rooting and acclimatization and enhance the production of true-to-type planting material.

An Unknown 18th-Century Flemish Dyers Manuscript from Antwerp (1778–1802)

This paper presents a historical analysis of a rare dyer’s manuscript, preserved within the Museum of Industry in Ghent, Belgium. The manuscript, originating from a dyer in late 18th-century Antwerp, includes an extensive collection of recipes. The study will enable researchers to better grasp the practices of traditional dyeing techniques and materials in the region during that time. The manuscript focuses primarily on the dyeing of woolen fabrics. Approximately 90 of the 132 recipes utilize red dyes. Recipes for dying orange, brown, black, blue, and green colors are also described. The document mentions the use of madder, brazilwood, redwood, and cochineal. To create a variety of red shades, the dyer describes how fabrics were treated with different mordanting compounds, with alum and tin as the main ingredients, and how the dyeing solutions were prepared. The resulting colors include ‘madder red’, ‘formal red’, ‘crimson’, ‘scarlet’, ‘Turkish red’, ‘fire color’ and ‘flesh color’. In addition to the dyeing recipes, the manuscript contains various accounting documents and correspondences between the dyer, customers, and suppliers. Lastly, over 100 original, colored samples are attached to the described recipes. In this paper, the artifact’s contents will be disclosed, comprising recipes with attached samples and correspondence. Findings resulting from archive research will be included, contextualizing and placing the dyer in their urban and social context. The paper concludes by discussing its potential limitations and provides avenues for possible future research.

Trends of the cannabis market in Poland over the last decade = Tendencje na rynku konopi indyjskich w ostatniej dekadzie w Polsce

Cannabis is the most widely used drug. in recent years, interest in cannabis has grown significantly. over the last decade, the number of cannabis users worldwide has increased by almost 21%, reaching approximately 219 million people in 2021. Can- nabis cultivation covers practically all regions of the world. In recent years, 151 countries have reported cannabis cultivation, both outdoors and indoors. The increased growth of the latter is particularly observed in North America and Europe. Global data shows that in 2021, 5,226 tons of marihuana and 2,014 tons of hashish were confiscated. an increase in seizures of can- nabis products is observed in Europe and Poland. Another trend concerns the increasing content of the psychoactive substance Δ9-tetrahydrocannabinol (Δ9-THC) in cannabis plants. According to UNODC data, over the last 25 years there has been a 4-fold increase in the content of Δ9-THc in cannabis. over the last decade, the average content of Δ9-tetrahydrocannabinol in marihua- na seized in europe has increased approximately twice, and in hashish three times. The aim of this work is to assess potential changes in the content of the main psychoactive ingredients in cannabis in Poland in the last decade (2012–2022), based on expert opinions developed by the Institute of Forensic research in Kraków. The conduct- ed analyses confirmed the trends observed around the world. in the last ten years, the number of cases in which the drug-type cannabis was detected increased from 5.3% to 68.4% of total number of illicit drug cases examined for the presence of narcotic drugs, psychotropic substances and new psychoactive substances. an increasing trend in the content of Δ9-THC in the drug-type cannabis was also noted. over the last decade, the average total content of Δ9-THC increased almost three times and in 2022 reached 12%. This is due to the growth of cannabis cultivation in Europe, carried out using the technique known as sinsemilla, genetic modifications, as well as the appearance on the market of the feminized cannabis seeds.

Roadside inspections, traffic events and traffic accidents from 2010–2022 in the experience of the Institute of Forensic Research as a consulting body in light of the proposed thresholds for the states of ‘after use’ and ‘under the influence’ for THC = Kontrole, zdarzenia i wypadki drogowe z lat 2010–2022 w praktyce opiniodawczej Instytutu Ekspertyz Sądowych w świetle propozycji progów „po

Road traffic safety depends largely on the psychophysical fitness of drivers. Apart from ethyl alcohol, this efficiency is influenced by many other factors, including: presence in the blood of the so-called substances that act similarly to alcohol, and cannabinols constitute one of the most popular groups of these substances. The paper presents statistics on delta-9-tetrahydrocannabinol (THC) – the main psychoactive ingredient of cannabis detected in the blood of drivers subjected to 7269 road inspections and involved in 4697 road events and accidents in the years 2010–2022 throughout Poland. Based on the results obtained, it was found that THC is present in the blood of 25–61% of roadside inspection cases, and in the case of road incidents and accidents, 5–15% of drivers have THC in a concentration equal to or higher than 1 ng/ml. The data in the charts are given in relation to two THC concentration ranges: 1.0–2.5 ng/ml and above 2.5 ng/ml (the value proposed as the limit of the state “after use” and “under the influence” of THC) and in relation to concentration ranges based on the THC elimination curve in human serum. The study did not distinguish between chronic or acute participants and occasional participants. It was based on THC and THCCOOH concentrations. The analyzes were performed using the enzyme-linked immunosorbent method (ELISA) and confirmatory methods such as liquid and gas chromatography coupled with mass spectrometry, in which the limit of quantification of 1 ng/ml was in accordance with the applicable requirement contained in the Regulation of the Minister of Health of July 16, 2014 on the list of similarly acting agents to alcohol and the conditions and method of conducting tests for their presence in the body.

Diversity in Pyracantha fortuneana fruits maturity stages enables discrepancy in the phenolic compounds, antioxidant activity, and tyrosinase inhibitory activity.

Pyracantha fortuneana (P. fortuneana) fruit is a wild fruit that is popular because of its delicious taste and numerous nutrients, and phenolic compounds are considered to be the main bioactive components in P. fortuneana fruits. However, the relationship between phenolic compounds and their antioxidant and tyrosinase (TYR) inhibitory activities during the ripening process is still unclear. The study compared the influence of the five developmental stages on the accumulation of phenolic compounds, antioxidant activity, and TYR inhibitory activity in the fruits of P. fortuneana. The compounds were identified by offline two-dimensional liquid chromatography-electrochemical detection (2D-LC-ECD) combined with liquid chromatography-tandem mass spectrometry, and the main active ingredients were quantified. The results showed that stage II had higher total phenolic and flavonoid content, as well as higher antioxidant and TYR inhibitory activity, but the total anthocyanin content was lowest at this stage. A total of 30 compounds were identified by 2D-LC-ECD. Orthogonal partial least squares discriminant analysis screened out six major potential markers, including phenolic acids, procyanidins, and flavonoids. In addition, it was found that caffeoylquinic acids, procyanidins, and flavonoids were higher in stage II than in stages I, III, IV, and V, whereas anthocyanins accumulated gradually from stages III to V. Therefore, this study suggests that the changes in antioxidant and TYR inhibitory activities of P. fortuneana during the five developmental stages may be due to the transformation of procyanidins, caffeoylquinic acids, and phenolic glycosides into other forms during the fruit maturation process. Practical Application: Differences in chemical constituents, antioxidant, and tyrosinase inhibitory activities in fruit maturity stages of P. fortuneana were elucidated to provide reference for rational harvesting and utilization of the fruits and their bioactive components. These findings are expected to provide a comprehensive assessment of the bioactive profile and guide the food industrial production.

New Cocrystals of Ligustrazine: Enhancing Hygroscopicity and Stability

Ligustrazine (TMP) is the main active ingredient extracted from Rhizoma Chuanxiong, which is used in the treatment of cardiovascular and cerebrovascular diseases, with the drawback of being unstable and readily sublimated. Cocrystal technology is an effective method to improve the stability of TMP. Three benzoic acid compounds including P-aminobenzoic acid (PABA), 3-Aminobenzoic acid (MABA), and 3,5-Dinitrobenzoic acid (DNBA) were chosen for co-crystallization with TMP. Three novel cocrystals were obtained, including TMP-PABA (1:2), TMP-MABA (1.5:1), and TMP-DNBA (0.5:1). Hygroscopicity was characterized by the dynamic vapor sorption (DVS) method. Three cocrystals significantly improved the hygroscopicity stability, and the mass change in TMP decreased from 25% to 1.64% (TMP-PABA), 0.12% (TMP-MABA), and 0.03% (TMP-DNBA) at 90% relative humidity. The melting points of the three cocrystals were all higher than TMP, among which the TMP-DNBA cocrystal had the highest melting point and showed the best stability in reducing hygroscopicity. Crystal structure analysis shows that the mesh-like structure formed by the O-H⋯N hydrogen bond in the TMP-DNBA cocrystal was the reason for improving the stability of TMP.

Critical intermittency in rational maps

Intermittent dynamics is characterized by long periods of different types of dynamical characteristics, for instance almost periodic dynamics alternated by chaotic dynamics. Critical intermittency is intermittent dynamics that can occur in iterated function systems, and involves a superattracting periodic orbit. This paper will provide and study examples of iterated function systems by two rational maps on the Riemann sphere that give rise to critical intermittency. The main ingredient for this is a superattracting fixed point for one map that is mapped onto a common repelling fixed point by the other map. We include a study of topological properties such as topological transitivity.

Deviation inequalities for dependent sequences with applications to strong approximations

In this paper, we give precise rates of convergence in the strong invariance principle for stationary sequences of bounded real-valued random variables satisfying weak dependence conditions. One of the main ingredients is a new Fuk-Nagaev type inequality for a class of weakly dependent sequences. We describe also several classes of processes to which our results apply.

The hawthorn (Crataegus pinnatifida Bge.) fruit as a new dietary source of bioactive ingredients with multiple beneficial functions

AbstractThe discovery of new natural sources has brought increased attention to the development of functional foods. The hawthorn (Crataegus pinnatifida Bge.) fruit is an underutilized fruit due to its benefits for human health and good taste. It contains a variety of bioactive ingredients, contributing to its multiple beneficial functions and applications. This review summarized and discussed the main bioactive ingredients, beneficial functions based on in vitro, in vivo, and human studies, and different applications of the hawthorn fruit according to the updated literature in the past 3 years. Hawthorn berries contain phenolic acids, flavonoids, proanthocyanidins, pectin, and many other bioactive components, which have a variety of beneficial functions, such as antioxidant, anti‐inflammatory, antibacterial, antidiabetic, intestinal protection, cardiovascular protection, hepatoprotection, anti‐cancer, and neuroprotection. Its potential molecular mechanism and different food‐related applications such as hawthorn wine and antioxidant drink are discussed in detail in this review. Additionally, hawthorn berries are shown to be safe when consumed within the proper dosage. Collectively, this updated review indicates that the hawthorn fruit can be a new dietary source of bioactive ingredients with multiple beneficial functions and can be affordably developed into functional and medicinal foods for the prevention and management of certain chronic diseases.

Assessment of Embedding Schemes in a Hybrid Machine Learning/Classical Potentials (ML/MM) Approach.

Machine learning (ML) methods have reached high accuracy levels for the prediction of in vacuo molecular properties. However, the simulation of large systems solely through ML methods (such as those based on neural network potentials) is still a challenge. In this context, one of the most promising frameworks for integrating ML schemes in the simulation of complex molecular systems are the so-called ML/MM methods. These multiscale approaches combine ML methods with classical force fields (MM), in the same spirit as the successful hybrid quantum mechanics-molecular mechanics methods (QM/MM). The key issue for such ML/MM methods is an adequate description of the coupling between the region of the system described by ML and the region described at the MM level. In the context of QM/MM schemes, the main ingredient of the interaction is electrostatic, and the state of the art is the so-called electrostatic-embedding. In this study, we analyze the quality of simpler mechanical embedding-based approaches, specifically focusing on their application within a ML/MM framework utilizing atomic partial charges derived in vacuo. Taking as reference electrostatic embedding calculations performed at a QM(DFT)/MM level, we explore different atomic charges schemes, as well as a polarization correction computed using atomic polarizabilites. Our benchmark data set comprises a set of about 80k small organic structures from the ANI-1x and ANI-2x databases, solvated in water. The results suggest that the minimal basis iterative stockholder (MBIS) atomic charges yield the best agreement with the reference coupling energy. Remarkable enhancements are achieved by including a simple polarization correction.

Developing and Assessing a Cream Designed to Remove Stretch Marks from “Prunus armeniaca” and “Centella asiatica”

Stretch marks (Striae distensae) are visible lines on the outer skin located usually on abdomen, breats, thighs. Stretch marks which develop on pregnant women known as striae gravidarum. Almost 50-90% of pregnant women may develop striae distensae. It is caused due to sudden increase in weight during pregnancy, due to rapid weight gain the collagen fibres present between dermis and epidermis layer of skin gets break down and stretch marks appears. Because of various side effects of synthetic medications, the use of herbal medications has increased. To avoid side effect, oil obtained from the leaves of Centella asiatica linn i.e. Gotukola and the fruits of Prunus armeniaca i.e. Apricot was incorporated into the formulation stimulates collagen synthesis by energizing dermal fibroblast and regenerate the skin cells. The main active ingredient found in gotukola is triterpenoids (saponin) and Apricot is glycosides, it increases collagen production in body and regenerate the skin cells this may help to prevent new stretch marks from forming as well as help to heal any existing marks. The objective of the present study was to developing the cream for the removal of stretch marks. Creams was prepared by oil-in-water emulsion by changing the concentration of active ingredients. Total 3 formulation were prepared among these F3 batch. In this (1.5% and 2%) concentration of oil was added and some butters were added. The final formulation shows pH (8.9), No skin irritancy, better spreadability, consistency and antimicrobial testing.

PSIV-A-13 The relationship between breed-wide expected progeny differences, genomic predictors, and phenotypic values in mature Angus cows

Abstract For many years, selection for improved beef production centered on heavier weaning weights and more rapid post-weaning gain. More recently, measurement of post-weaning feed intake has facilitated direct improvement in feed efficiency in the feedlot. However, intake on a high concentrate diet is controlled by different biological mechanisms than intake on a high roughage diet. For this reason, the question remains whether an animal that efficiently converts calories from a high-quality concentrate-based ration similar to that of a feedlot diet where the expected progeny differences (EPDs) and genomic predictors (GPs) are developed would also be an efficient forage utilizer as a mature cow. To evaluate this relationship, we compared breed-wide EPDs and predictors to performance on a roughage diet and on a concentrate diet in mature cows. The objective of this study was to determine if EPDs and GPs developed and used in feedlots are applicable to commercial cow/calf producers utilizing forages and other roughages as the main diet ingredient. Data for this study were collected at Oklahoma State University from the fall of 2018 to the spring of 2021. Tissue samples from 64 mature Angus cows were submitted to Neogen for profiling according to genetic potential for 16 different traits. In each trial, retained energy (RE), dry matter intake (DMI), milk production (MILK), mature weight (MW), and average daily gain (ADG) were recorded and compared with the corresponding EPD and GP. Pearson correlations were determined between phenotypic values, GPs, and EPDs for both diets on each trial. No EPD or GP was significantly correlated to any measured trait on the forage diet. The DMI EPD was strongly correlated with DMI on a concentrate-based diet, however, did not predict DMI on a forage diet in mature cows. Based on the evaluations performed in this study, selecting for animals that perform well in the feedlot may be inadvertently selecting for mature cows that perform poorly on a typical mature cow diet of medium to low-quality forages.

158 Nutrient digestibility in growing pigs fed a corn distiller’s dried grains with soluble-based diet with multi-enzyme supplementation

Abstract Dietary enzyme supplementation may enhance the digestibility and utilization of non-starch components in fiber-rich feedtuffs which are increasingly used in swine diets. A xylanase/cellulase enzyme product (ENZ) was previously shown to increase the digestibility of nutrients and energy in growing pigs fed a corn-SBM diet with 30% corn dried distiller’s grains with solubles (cDDGS). To better elucidate the effects of ENZ on these components, further analysis was conducted on the fiber, non-starch polysaccharide, and ash components. A total of 12 ileal-cannulated pigs [initial body weight (BW) = 31.7 kg ± 3.04] were housed in individual metabolic crates for 21 d (period 1) and subsequently transferred to individual floor pens for an additional 28 d (period 2). These animals were assigned to 2 diets (n = 6) in a completely randomized design (CRD). The diets contained the main ingredients corn, soybean meal and cDDGS (30%) without (CON) or with ENZ supplementation. The inclusion rate of the ENZ (proprietary xylanase/cellulase product, Kerry Inc.) was 200 g/ton. Feed was formulated to 1.15% SID lysine and met or exceeded nutrient requirements for growing pigs (NRC, 2012). The diets were fed to pigs at three times maintenance energy requirement based on BW, which was assessed at 2 wk intervals throughout the study. The diets contained 0.3% titanium dioxide as an indigestible marker. In period 1, pigs had 14 d of adaptation, followed by 4 d of fecal and urine collection using the total collection method and 2 d of ileal digesta collection. In period 2, pigs had 23 days of adaptation, followed by 2 d of fecal collection and 2 d of ileal digesta collection. Data were analyzed as a CRD, using pig as the experimental unit and enzyme supplementation as the main effect. The ATTD of total NSP and galactose tended to improve in both periods 1 and 2. As consistent responses were observed in periods 1 and 2 and the ENZ X period interaction was not significant, the data from these time periods were combined for analysis. ENZ supplementation improved (P < 0.05) the ATTD of total NSP and galactose. Also, ENZ tended to improve (P < 0.10) the ATTD of cellulose and glucose from NSP. ENZ addition also increased the ATTD of calcium (P < 0.05) and tended to increase the ATTD of phosphorus (P < 0.10). In conclusion, supplementation of a corn-SBM-30% cDDGS diet with a multi-carbohydrase containing cellulase increased the ATTD of fiber, NSP and calcium in growing pigs. Novel enzymes represent a useful strategy to better utilize the nutrients in lower energy feedstuffs in monogastric animals.

Triptolide regulates neutrophil function through the Hippo signaling pathway to alleviate rheumatoid arthritis disease progression

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by inflammatory changes in the joints, the etiology of which is unclear. It is now well established that regulated cell death (RCD) and migration of neutrophils play an important role in the pathogenesis of RA. Tripterygium wilfordii Hook.f (TwHF) is a total saponin extracted from the root of Tripterygium wilfordii Hook.f, a plant of the family Wesleyanaceae, which has strong anti-inflammatory and immunomodulatory effects and has been used as a basic drug in the clinical treatment of RA. Despite the good efficacy of TwHF treatment, the mechanism of action of TwHF remains unclear. Several studies have demonstrated that the drug tripterygium glycosides, in which TwHF is the main ingredient, has achieved excellent efficacy in the clinical treatment of RA. Investigations have also found that TwHF can affect cellular RCD, cell migration, cell proliferation, and the apoptosis-related Hippo signaling pathway. In this study, we first analyzed the RCD and migration differences of neutrophils in patients with RA through network pharmacology and transcriptome analysis. Subsequently, we used electron microscopy, immunofluorescence, and other methods to identify the RCD phenotype of neutrophils. In collagen-induced arthritis (CIA) model, we demonstrated that Triptolide (the main active ingredient in TwHF) could alleviate the progression of arthritis by reducing the bone destruction and the infiltration of neutrophils. Furthermore, in vitro experiments showed that Triptolide induced neutrophil apoptosis, inhibited the formation of neutrophil extracellular traps (NETs), and impeded the neutrophil migration process in a Hippo pathway-dependent manner. Taken together, these findings indicate that Triptolide has potential for treating RA and provide theoretical support for the clinical application of TwHF, as a traditional Chinese medicine, in RA.

Wegner estimate and upper bound on the eigenvalue condition number of non‐Hermitian random matrices

AbstractWe consider non‐Hermitian random matrices of the form , where is a general deterministic matrix and consists of independent entries with zero mean, unit variance, and bounded densities. For this ensemble, we prove (i) a Wegner estimate, that is, that the local density of eigenvalues is bounded by and (ii) that the expected condition number of any bulk eigenvalue is bounded by ; both results are optimal up to the factor . The latter result complements the very recent matching lower bound obtained by Cipolloni et al. and improves the ‐dependence of the upper bounds by Banks et al. and Jain et al. Our main ingredient, a near‐optimal lower tail estimate for the small singular values of , is of independent interest.

Expression of RIPK1 and FADD are associated with chemosensitivity and survival in head and heck squamous cell carcinoma via tanshinone IIA-mediated modulation of the RIPK1-FADD-Caspase 8 complex.

Tanshinone IIA (Tan IIA), a main active ingredient of salvia miltiorrhiza, has a wide range of antitumor effects, while its specific role and mechanism in head and neck squamous cell carcinomas (HNSCC) is not fully understood. Totally 59 primary HNSCC patients underwent two courses of induction chemotherapy before surgery. The association between expression of Fas-Associated Death Domain (FADD) and receptor interacting protein kinase 1 (RIPK1) and chemotherapy resistance and survival were evaluated. The cell counting kit-8 was used to detect the effect of Tan IIA on the activity of cisplatin in chemoresistant HNSCC cells through a series of in vitro experiments. The quantitative real-time reverse-transcription polymerase chain reaction, Western blot analysis and flow cytometry were used. FADD and RIPK1 expressions were differentially expressed in Chemosensitive and drug-resistant patients. Furthermore, patients with tumors exhibiting high expression of FADD and RIPK1 had significantly greater risk for chemoresistance and mortality than patients with tumors that had low levels of these proteins. Moreover, Tan IIA reduced the expression of RIPK1 and FADD in HNSCC chemoresistant cell lines, which could increase the chemosensitivity of cisplatin and promote apoptosis. Overexpression of RIPK1 led to attenuation of therapeutic effects of Tan IIA, which were mainly realized through regulation of the RIPK1-FADD-Caspase 8 complex. This study is the first to demonstrate the clinical value and role of FADD and RIPK1 in the treatment of HNSCC. This work establishes the proapoptotic effects of Tan IIA and its potential to enhance chemosensitivity in HNSCC by modulating the RIPK1-FADD-Caspase 8 complex.

Decay estimates for defocusing energy-critical Hartree equation

Abstract In this paper, we are devoted to establishing the point-wise decay estimates for solution to the 5D defocusing energy-critical Hartree equation with an initial data in H 2 ( R 5 ) ∩ L 1 ( R 5 ) ${H}^{2}\left({\mathbb{R}}^{5}\right)\cap {L}^{1}\left({\mathbb{R}}^{5}\right)$ . We show that the nonlinear solution has the same time decay rate as the linear one. The main new ingredient is that we used the theories of Lorentz spaces to overcome the low power of nonlinearity.

Application of the kano model to identify important sensory attributes of bubble tea drinks accompanied with boba pearl made from purple sweet potato and sugar palm fruit

The Indonesian bubble tea market is experiencing rapid growth and continuous innovation, marked by a diverse array of flavors and conceptual approaches. Bubble tea commonly includes tapioca pearls, rich in carbohydrates but lacking essential nutrients. To create healthier boba pearls, alternative ingredients like sweet purple potato and sugar palm fruit could be utilized. However, the critical sensory attributes influencing consumer preferences for these boba pearls remain unidentified. This research aims to discern essential attributes in bubble tea drinks with boba pearls made from purple sweet potatoes and sugar palm fruit, utilizing the Kano model to identify influential attributes. The KANO survey identified several attractive attributes, including the use of purple sweet potatoes and sugar palm fruit as main ingredients, the natural purple color, aroma, and taste of purple sweet potatoes, vanilla aroma and taste, utilization of cassava fructose syrup, creaminess in the bubble tea drink, and a naturally sweet taste from cassava fructose syrup. These attractive attributes demonstrated customer satisfaction coefficients ranging from 0.53 to 0.82 and dissatisfaction coefficients from -0.03 to 0.05. These findings are valuable for prioritizing attributes in the development of appealing and healthier bubble tea beverages, addressing consumer preferences effectively.